INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
The carbohydrate-binding module of Fragaria x ananassa expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities ?"in vitro"..
Autor/es:
NARDI CRISTINA F.; ESCUDERO CRISTIAN M.; VILLARREAL N.; MARTÍNEZ GUSTAVO A.; CIVELLO P.
Revista:
JOURNAL OF PLANT RESEARCH
Editorial:
SPRINGER TOKYO
Referencias:
Lugar: Tokyo; Año: 2012
ISSN:
0918-9440
Resumen:
Abstract A putative carbohydrate binding module (CBM) from strawberry (Fragaria x ananassa Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in Escherichia coli and purified in order to evaluate its capacity to bind different cell wall polysaccharides ??in vitro??. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (Kad = 33.6 ± 0.44 mL g-1, Kad = 11.37 ± 0.87 mL g-1, Kad = 10.4 ± 0.19 mL g-1, respectively). According to ??in vitro?? enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBMFaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers.
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