INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
Autor/es:
SMY LOPEZ1, PEDRO A. BALATTI
Revista:
Biotechnology Journal Genetic Engineering and Biotechnology
Editorial:
Aston Journals
Referencias:
Año: 2012 vol. 34 p. 1 - 1
ISSN:
2150-3516
Resumen:
The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSá successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSá sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
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