INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
Exposure to Nitric Oxide Increases the Nitrosyl-Iron Complexes Content in Sorghum Embryonic Axes.
SIMONTACCHI M; BUET A; LAMATTINA L. ; PUNTARULO S
ELSEVIER IRELAND LTD
Lugar: Amsterdam; Año: 2012 vol. 183 p. 159 - 159
Background This work was aimed to investigate nitrosyl-Fe complexes formation by reaction of endogenous ligands and Fe, in sorghum embryonic axes exposed to NO-donors. Methods Electron paramagnetic resonance (EPR) was employed to detect the presence of nitrosyl-Fe complexes in plant embryos, as well as changes in labile iron pool (LIP). Results Nitrosyl-Fe complexes formation was detected in sorghum embryonic axes homogenates incubated in vitro in the presence of 1 mM of the NO donors. In axes isolated from seeds incubated in vivo in the presence of 1 mM SNP for 24 h, the content of endogenous NO was increased by 2-fold, and the EPR spectrum from MNIC complexes was observed with a concomitant increase in the fresh weight of sorghum axes. The simultaneous exposure to DFO and NO donor, precluded the increase in fresh weight observed in the presence of excess NO. While total Fe content in the axes isolated from seeds exposed to 1 mM SNP was not significantly affected as compared to control axes, the LIP was increased by over 2-fold. Conclusion and general significance The data reported suggest a critical role for the generation of complexes between Fe and NO when cells faced a situation leading to a significant increase in NO content. Moreover, demonstrate the presence of MNICs as one of the important components of the LIP, which could actively participate in Fe cellular mobilization.