INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
UV-C treatment affects the expression and activity of defense genes in strawberry fruit (Fragaria x ananassa, Duch.)
POMBO MARINA; ROSLI HERNAN; MARTINEZ GUSTAVO; CIVELLO PEDRO
POSTHARVEST BIOLOGY AND TECHNOLOGY
ELSEVIER SCIENCE BV
Año: 2011 vol. 59 p. 94 - 94
UV-C treatment is a newand environment-friendly strategy to reduce postharvest decay in several fruits. In this work, we studied the induction of resistance to Botrytis cinerea in strawberry fruit, exposed to a hormetic dose of UV-C. The results obtained showed that pre-storage treatment of fruitwith UV-C results in lower losses caused by diseases and decay. Therefore,we analyzed the gene expression and enzymatic activity of a set of strawberry genes that are related to plant defense against pathogens. The expression of the genes and the activity of assayed enzymes were modiﬁed in the treated fruit. The expressionand enzyme activity of phenylalanine ammonia lyase (PAL, EC: 220.127.116.11) increased over the level found in control after 4 and 24 h of storage. The activity of -1,3-glucanase (EC: 18.104.22.168) and peroxidase (EC:22.214.171.124) showed a biphasic pattern. Higher polyphenol oxidase (PPO, EC: 126.96.36.199) activity was observed in treated fruit comparedwith control from10 to 48 h post-irradiation. Moreover, we studied the expression of several strawberry PR proteins.We found an induction of FaChi2-2 and FaChi3 immediately afterthe treatment, and the corresponding chitinase (EC: 188.8.131.52) activity increased 10 h post-treatment. Gene expression proﬁle of -1,3-glucanaseswas different fromthose of chitinases. FaBG2-1mRNA levels increased in the treated fruit during the beginning of storage, but in the case of FaBG2-3, irradiated fruit showed higher expression than the control after 24 and 48 h of storage. Finally, FaPR1 gene expression was stimulated by UV-C treatment at 4 and 24 h of storage. No changes in FaOLP2 expression in fruitwerefound in response to irradiation. Therefore, the reduction in strawberry fruit decay by UV-C treatment at harvest could be related to the increase in the transcription and activity of a set of enzymes and proteins involved in the defense against pathogens.