Antibiofilm activity of the ternary cadmium complex with 1,10-phenanthroline and cyanoguanidine against Pseudomonas aeruginosa

MARTÍNEZ MEDINA, J.J.; WILLIAMS, P.A.M.; LOPEZ TEVEZ, L.; D. FALKIEVICH; FERRER, EVELINA G.

Encuentro; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1) EVENTO VIRTUAL; 2021

docentes investigadores de las Universidades Nacionales de Córdoba y Rosario

In recent years, infections caused byantimicrobial-resistant strains have become a global health challenge. Pseudomonas aeruginosa is a pathogenwhich causes infections in immunocompromised patients. Control of this pathogenhas become increasingly difficult due to its adaptive resistance mechanisms, likebiofilm-mediated resistance. A biofilm is an aggregate of microorganisms thatadhere to each other on a living or non-living surface. Previously, we determinedthe minimum inhibitory concentration of the [Cd(1,10-phenanthroline)2(SO4)H2O]cyanoguanidine?5H2Ocomplex (187 μg/mL), cyanoguanidine (>1000 μg/mL), and the ligandphenanthroline (375 μg/mL) against P.aeruginosa ATCC 27853. Besides, the Artemiasalina test showed an improved safety profile for the complex. In thiswork, we evaluated the antibiofilm activity of the cadmium complex and theligand phenanthroline against this bacterial strain using the crystal violetassay (pre- and post-adhesion assays) in 96-well microtiter plates. The complexshowed dose-dependent antibiofilm activity, inhibiting the biofilm formationclose to 25%, 70% and 80% in the pre-adhesion assay (20%, 50% and 55% in thepost-adhesion assay) at 0.25, 0.5 and 1 MIC values, respectively. The ligand phenanthrolineshowed a similar behavior. In summary, the complexation is a powerful tool fordesign alternative antimicrobial drugs active against biofilm-forming strainsand with an improved safety profile.