CEQUINOR   05415
CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IN VITRO ANTITUMOR EFFECTS OF TWO COPPER COMPLEXES WITH PHENANTROLINES DERIVATIVES TOWARD 2D AND 3D BONE, LUNG AND BREAST CANCER CELL MODELS.
Autor/es:
LEMUS CHAVEZ L.A.; ETCHEVERRY S.B.; RUIZ, M.C.; DI VIRGILIO A.L.; CADAVID VARGAS J.F.; LEON, I.E
Lugar:
Mar del Plata
Reunión:
Congreso; SAIC-SAI-SAFIS; 2018
Resumen:
Several findings provide evidence that copper ions are capable of interacting directly with proteins and DNA, causing site-specific damage. It has been reported that copper compounds increase cell death in different cancer cell lines and both, the ligand and the metal play an important role in the pharmacological properties of the complex. During this work, we attempt to evaluate the biological activity of two different copper complexes, CuII(dmp)2(CH3CN)](ClO4)2 (1) and CuII(phen)2](ClO4) (2) against cancer cells lines: A549 (lung), MG-63 (osteosarcoma), MCF7 and MDA-MB-231 (breast cancer). On monolayer, cell viability was analyzed by MTT, the cell migration was evaluated by wound healing assay, the ROS production was quantify using DHR123 and the genototoxic activity was determinate using comet assay. Besides, the cell viability of the spheroids (A549, MCF7 and MG-63) was evaluated by alamar blue assay. Complex 1 proved to be more active than complex 2 in all the cell lines tested, showing the following IC50 values of complexes 1 and 2 respectively, A549 1.94 µM vs 3.34 µM, MG-63 3.07 µM vs 3.11 µM, MDA-MB-231 1.95 µM vs 5.11 µM and MCF7 2.99 µM vs 6.11 µM. (p