Cyto- and genotoxicity of a Ruthenium-clioquinol complex against 2D and 3D human osteosarcoma models.

KLJUN J.; LEON I.E; CADAVID-VARGAS J.F; TUREL I; RUIZ M.C; DI VIRGILIO A.L; ETCHEVERRY S.B

Montevideo

Congreso; X Congreso de ALAMCTA; 2016

The aim of this project is to determine the cyto- and genotoxic activity of the Ru (Cym-CQCl) in cellular monolayers models and in spherical 3D models using MG-63 cells.The cytotoxicity of the compound was evaluated with MTT assay. Concerning the genotoxic potential, this was evaluated using the comet and micronucleus techniques. Besides it was evaluated the nuclease activity of the complex like as another genotoxicity parameter. of genotoxicity Besides, the antitumor properties of the Ru compound on 3D model were performed through the circularity measure and cell viability using acid phosphatase assay.The MTT assay demonstrates that the complex impairs the metabolism of mitochondria. It is reflected on the cell viability, The compound the complex decrease the cell viability in a 50% after 24 hours of incubation with 25 µM and at 50 µM the cell viability was less than 40%. The data also show that CQ decreased the viability around 20%, proving the biological effects of complexing this ligand with ruthenium.The complex exhibited nuclease activity toward pA1. the pA1 DNA showed increased migration in agarose gel. The genotoxic process was further shown through increased tail moment and the formation of micronucleus in a concentration range of 2.5 µM to 10 µM. It be assumed that the compound shows the ability to induce DNA damage in these cells. Moreover, the spheroid diminished their spherical shape, and the viability of these cells appeared to be 30% less. The results imply that the complex has a potent anti?tumor activity in this model, as it reduced the size of the spheroid affecting the cell viability.