Antitumor Properties and Nuclease Activity of Platinum(II) Complexes Based on Hidroxyquinoline Ligands in a Human Osteosarcoma Cell Line and Plasmid DNA

LEON I.E; BUTENKO N; CADAVID-VARGAS J.F; DI VIRGILIO A.L; MARTIN SANTOS CECILIA; ALEMAN JOSE; NAVARRO RANNINGER C; ETCHEVERRY S.B

Wrocław

Simposio; International Symposium on Metal Complexes (ISMEC 2015); 2015

Despite the remarkable success of CDDP (cisplatin) [1] in the treatment of different types of cancers, two major limitations of the drug are of concern (i) its dose-limiting effects and (ii) inactivity against some of the most frequent human tumour types. Thus, the necessity for the search of structural analogues endowed with fewer side effects and capable of overcoming acquired resistance to CDDP has arisen. The hydroxyquinolines derivatives have medicinal properties such as antineurodegenerative, anti-inflammatory, and anticancer activities [2]. In this project we studied the anticancer properties, mechanism of action and nuclease activity of two platinum(II) complexes (1 and 2) with analogous of quinolines in a human osteosarcoma cell line (MG-63) and with plasmid DNA (pDNA, pA1). Based on the results of proliferation and cytotoxic assays (crystal violet and MTT assays) it was observed that after 6 h complex 2 caused a cell viability inhibition at 10-100 µM while 1 did not show any effects in the same range of concentrations (p