Influence of 45s5 bioglass® concentration on vancomycin hydrochloride release from gelatinstarch films. Evaluation of antibacterial effects

RIVADENEIRA J.; DI VIRGILIO A.L.; AUDISIO M.C.; BOCCACCINI A.R.; GORUSTOVICH A.A.

Workshop; II Workshop on Bio-degradable Polymers and Biocomposites. III Workshop BIOPURFIL, Bio-based Polyurethane Composites with Natural Fillers.; 2015

One important factor that interrupts the tissue repair is bacterial burden in the wound. Starch can be used together with gelatin as reinforcement to the polymeric matrix and for controlled release drug delivery [1]. 45S5 bioactive glass (BG; 45% SiO2, 24.5% Na2O, 24.5% CaO, 6%P2O5) is a promising material for application in soft tissue engineering as it possesses several biological effects [2]. The aim of this work was to investigate the influence of 45S5 BG microparticles (m-BG) on the degradation, release profile of the antibiotic vancomycin hydrochloride (VC) and in vitro antistaphylococci effects of gelatin-starch (GS) composites.Composites were fabricated through gel casting methodology. Four different composites were prepared according to the percentage of m-BG: 0%, 5%, 10% and 15%. VC was added to this composite at concentration of 0.5 mg mL-1. Biodegradation were performed by soaking the composites in PBS. Drug release kinetics was quantified spectrophotometrically at 280 nm. The antibacterial effects were evaluated on Staphylococcus aureus ATCC29213, ATCC6538 and Staphylococcus epidermidis ATCC12228 by plate count method.The water uptake capacities were in the order of 250?300% with no differences in the swelling between the composites (Fig. 1A). The weight loss of GS films increase on increasing m-BG content (Fig. 1B) in agreement with previous results [3]. The release profiles of VC were modulated by crescent concentration of m-BG (Fig. 1C) in concordance with previous authors [4]. However, this modulation did not reflect an improvement inthe antibacterial effects of the composites. All composites strongly inhibited staphylococci cells with similar strength (Fig. 1D) making gelatin-starch/m-BG composites attractive matrices for wound dressing application.