Anticancer drug development. Effects of a complex of oxidovanadium(IV) with silibinin in osteoblast cell lines. Relationship with the inhibition of topoisomerase IB

LEÓN IE; PORRO, V; A.L. DI VIRGILIO; BOLLATI-FOGOLÍN M.; CASTELLI S.; DESIDERI A.; ETCHEVERRY SB.

Padova

Simposio; 9 th International Vanadium Symposium Chemistry, Biological Chemistry & Toxicology; 2014

University of Padova

Anticancer drug development. Effects of a complex of oxidovanadium(IV) with silibinin in osteoblast cell lines. Relationship with the inhibition of topoisomerase IB I.E. León1, V. Porro2, A.L. Di Virgilio1, M. Bollati-Fogolin2, S. Castelli3, A. Desideri3, and S.B. Etcheverry1 1Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina, 2Instituto Pasteur, Montevideo, Uruguay, 3 Universidad de Roma 2,Roma, Italy e-mail: etcheverry@biol.unlp.edu.ar The family of polyphenolic flavonoids as well as vanadium compounds present interesting pharmacological actions. In this work we report the biological effects of a complex of vanadyl(IV) with Silibinin Na2[VO(silibinin)2].6H2O (VOsilibinin) on MG-63 human osteosarcoma derived cell line and the results were compared with previous ones from our laboratory on two murine osteoblastic cell lines: MC3T3-E1 and UMR106. The effects on cell viability (crystal violet bioassay, MTT bioassay and neutral red uptake) and on the genotoxicity (micronucleus and comet assay) Showed that VOsilibinin caused an inhibitory effect on cell viability in a concentration-dependent manner (p < 0.01. The antiproliferative action of the complex was much stronger than that of the free flavonoid (p