Bioactivity of a new polyoxometalate with copper. Anti-tumoral activity in a human osteosarcoma model

LÓN, IE; DI VIRGILIO, A.L.; PATRICIA A.M. WILLIAMSRRO, V; EGUSQUIZA, G; CABELLO, C.; BOLLATI-FOGOLIN, M; ETCHEVERRY, S.B.

Punta del Esye

Simposio; 12th International Symposium on Metal Ions in Biology and Medicine; 2013

The polyoxometalates are transition metals clusters of oxide anions, usually d0 species (V(V), Mo(VI) y W (VI)) formed by a self assembly process. In addition, the heteropolyoxometalates (HPOMs) contain one or more heteroatoms with electrons of valency p-, d - and f. The structural and electronic properties of this type of compounds that are easy to modify and their characteristics makes them attractive for several applications in different fields, especially as pharmaceutical drugs. The biomedical importance of the POMs is based principally on its interaction with proteins, including enzymes and constituents of the cellular systems. The selected heteropolytungstate belongs to an isomorfic serie [(PW9O34)2M4]10- with M=Cu(II), was synthesized and completely characterized in our laboratories. In the frame of a research project devoted to study the potential pharmacological effects of heteropolytungstates with copper ([(PW9O34)2Cu4]10-), we present herein the results of its biological effects in an osteosarcoma human model. The effects on cell proliferation, mitochondria and lysosomal activity and morphology were investigated. Besides, the mechanisms of action involved in the cytotoxicity were also studied (oxidative stress, level of GSH, GSH/GSSG ratio and Cell cycle arrest). [(PW9O34)2Cu4]10- caused inhibition of cell proliferation in the range of 25-100 oM (p