Cyto and genotoxic effects of a complex of Cu(II) with Norfloxacin in cell culture. Theoretical study.

DI VIRGILIO, ANA LAURA; LEÓN, IGNACIO ESTEBAN; FRANCA, CARLOS ALBERTO; ETCHEVERRY, SUSANA BEATRIZ

Rosario

Congreso; XV Reunióm de la Sociedad Latinoamerican de Genétiva, XV! Reunión de la Sociedad Argentina de Genética; 2012

Norfloxacin is a member of the fluoroquinolone group of antibiotics used in the treatment of bacterial infections. Interactions between quinolone-metal complexes and DNA can be responsible for pharmacological effects on mammalian cells. Therefore, a deeper insight into the biological properties of these complexes is important for a better understanding of their therapeutic efficacy. We studied the potential antitumoral action of a complex of Norfloxacin with Cu(II), Cu(Nor)2.5H2O on rat osteosarcoma (UMR106) and mouse calvaria derived (MC3T3-E1) cells, evaluating its cyto- and genotoxicity. By a theoretical study, we have also elucidated the more stable conformation of the complex under physiological conditions. Cu(Nor)2.5H2O caused an inhibitory effect on the proliferation of both cell lines from 300 μM (p