CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
congresos y reuniones científicas
Image cell-based assays for vanadium-complexes antitumoral activity characterization
M.VALCARCEL; P. VILLACÉ; M. ROURA; D. KORTAZAR; C. SALADO; L. NASO; E.G. FERRER; P.A.M. WILLIAMS
Conferencia; Society for Laboratory Automation and Screening (SLAS2012); 2012
Society for Laboratory Automation and Screening
Vanadium compounds are known for a variety of pharmacological properties including their antitumoral activity. For this reason new VO(IV)-flavonoid complexes were generated and their cytotoxic effects were determined using different human breast cancer cell lines. Our results indicated that treatment with VO(IV)-flavonoid complexes affected cell viability of MDA-MD231, MDA-MB468, BT474, T47D and SKBR3 breast cancer cell lines. To further understand the pathway by which vanadium complexes induced apoptosis, different image cell based assays were developed. The assays were intended to explore apoptosis induced via mitochondrial pathway (Mitochondrial apoptosis assay cell line), Caspase activation (Caspase 3/7 activity assay cell line) or mitotic arrest (α-tubulin mitosis assay cell line). It is well known that cytochrome c is normally present inside the mitochondria. However, upon induction of apoptosis, mitochondrial membrane potential is perturbed, which results in release of cytochrome c from the mitochondria to cytosol. This process leads to the activation of caspase-9 followed by the activation of caspase 3/7 correlated with the result of apoptotic cell death. Our results indicated that VO(IV)-flavonoid complexes stimulated the apoptotic process in various steps along the death signalling pathway. Some of them triggered mitochondria mediated apoptosis whereas others caused apoptosis activation by blocking cell cycle. Thus, the utilization of these image cell-based assays allowed us to characterize the cytotoxic effects of new drugs for cancer treatment.