Antimicrobial properties of the ternary Zn(II) complex with sulfadiazine and 2,2′-bipyridine as ligands

CRISTIAN VILLA-PÉREZ; DANA , DANA B. FALKIEVICH; JUAN J. MARTINEZ MEDINA; DELIA BEATRIZ SORIA

Cordoba

Congreso; Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); 2022

6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1)

Abstract: Sulfadiazine (SDZ) is a bacteriostatic sulfonamide which inhibits the enzymatic activity of dihydropteroate synthetase, thus blocking the folic acid synthesis in bacterial cells. 2,2′-bipyridine (bipy) is a N,N′-bidentate ligand widely used as a coligand which has a broad antimicrobial spectrum. The antimicrobial profile of these compounds could be enhanced upon complexation with antiseptic metals like zinc. The aim of this work was to evaluate the antimicrobial properties of the novel Zn(II)SDZbipy complex. The minimum inhibitory concentration (MIC) was determined against five bacterial strains and seven fungal strains of Candida genus by the agar dilution method. Briefly, the microbial suspensions were inoculated onto the plates containing Mueller Hinton agar with different concentrations of the tested compounds (3.9 - 1000 μg/mL) and incubated aerobically at 37 °C for 24 and 48 h for bacteria and fungi, respectively. The Zn(II)SDZbipy complex showed MIC values from 125 to 500 μg/mL (clinically relevant) against Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Candida albicans, Candida glabrata and Candida krusei. Besides, the complexation enhanced the antimicrobial activity of SDZ against Escherichia coli, and the Staphylococcus and Candida strains mentioned above. In conclusion, the complexation of conventional chemotherapeutic agents is a powerful tool for design alternative broad-spectrum antimicrobials.