Interactions of rutin with the oxidovanadium (IV) cation. Anticancer improvement effects of glycosylated flavonoids

QUISPE, PATRICIA; REY, MARILIN; WILLIAMS, PATRICIA A. M.; NASO, LUCIANA G.; RIZZI, ALBERTO C.; GOITIA, HELEN; MARTÍNEZ, VALERIA R.; FERRER, EVELINA G.

NEW JOURNAL OF CHEMISTRY

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2019 p. 17636 - 17646

1144-0546

This work reports the biological evaluation of the new complex Na2[VO(rut)(OH)2]5H2O (rut = rutin, aglycosylated flavonoid). The complex was different in its coordination mode (catechol-like) from thosepreviously reported, [VO(rutin)(H2O)2]2(SO4)4H2O and [VO(rut)2]4H2O (acetylacetonate-like coordination).Due to the coordination mode, the complex only improved the antioxidant activity of the ligand againstsuperoxide and hydroxyl radicals. The results show that while both 100 mM rutin and V(IV)O did not exhibitcytotoxic activity on A549 cells, the complex selectively improved the anticancer effect (IC50 = 95 mM),cellular reactive oxygen species (ROS) generation and depletion of the non-enzymatic antioxidantglutathione (GSH), producing oxidized glutathione (GSSG), and it did not affect the viability of the normalembryonic lung cell line (MRC-5) (up to 100 mM). All these data, and given the reversion of the cell killingeffect of the complex upon treatment with the antioxidant agent N-acetyl-L-cysteine (NAC), suggested anoxidative stress mechanism. Rutin and VOrut can spontaneously bind bovine serum albumin (BSA) and theycan be stored and transported by the protein.