CEQUINOR   05415
CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
artículos
Título:
Potential bio-protective effect of copper compounds: mimicking SOD and peroxidases enzymes and inhibiting acid phosphatase as a target for anti-osteoporotic chemotherapeutics
Autor/es:
PARENTE, JULIANA E.; ALBERTO RIZZI; MARTINI NANCY; MARILIN REY; FERRER, EVELINA G.; FRANCO D´ALESSANDRO; WILLIAMS, PATRICIA A.M.
Revista:
MOLECULAR BIOLOGY REPORTS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2019 p. 867 - 885
ISSN:
0301-4851
Resumen:
Copper complexes with transformed methimazole ligand have been synthesized and characterized by elemental analysis,conductivity measurements, thermogravimetric analysis, EPR, FTIR and UV?Vis spectroscopies. Results support theirstoichiometries and geometrical structures: [Cu(C4H5N2S)2Cl2]·2H2O(1), [Cu(C8H10N4S)SO4H2O](2) and [Cu(C8H10N4S)SO4](3). ((C4H5N2)2S: bis(l-methylimidazol-2-yl)sulfide; (C4H5N2S)2 = Bis[bis(l-methylimidazol-2-yl)disulfide]) Concurrently, the structurally distinct soluble species corresponding to complexes (1) and (2) were subsequently used in an in vitroinvestigation of their potential biological properties. In view of their possible pharmaceutical activity, the complexes werein vitro evaluated as phosphatase acid inhibitors. Their radical bio-protective effects were also studied measuring the effectagainst DPPH? and O2?− radicals. Additional catalytic properties as peroxidase mimics were evaluated using Michaelis?Menten kinetic model by means of phenol red and pyrogallol assays. The complexes exhibited catalytic bromination activityand the ability to oxidize pyrogallol substrate indicating that they can be considered as functional models. The relationshipsbetween the structures and the in vitro biological activities have also been considered. Serum protein albumin has attractedthe greatest interest as drug carrier and the affinity of biological/pharmaceutical compound is relevant to the developmentof new medicine. In that sense, interaction studies by fluorescence and EPR spectroscopies were performed showing thebinding capacity of the complexes.