CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
Antioxidant, anticancer activities and mechanistic studies of the flavone glycoside diosmin and its oxidovanadium(IV) complex. Interactions with bovine serum albumin.
V.R. MARTÍNEZ; L.G. NASO; M. VALCARCEL; C. SALADO; P.A.M. WILLIAMS; L. LEZAMA; E.G. FERRER
BIOORGANIC & MEDICINAL CHEMISTRY.
PERGAMON-ELSEVIER SCIENCE LTD
Lugar: Amsterdam; Año: 2016 vol. 24 p. 4108 - 4108
The natural antioxidant flavonoid diosmin, found in citric fruits, showed low antioxidant properties among other flavonoids due to its structural characteristics and low cytotoxicity against lung (A549)and breast (T47D, SKBR3 and MDAMB231) cancer cell lines. The anticancer behavior has been improvedby the metal complex generated with the flavonoid and the oxidovanadium(IV) ion. This new complex,[VO(dios)(OH)3]Na56H2O (VOdios), has been synthesized and characterized both in solid and solution states. The interaction of the metal ion through the sugar moiety of diosmin precluded the improvement of the antioxidant effects. However, the cell-killing effects tested in human lung A549 and breast T47D, SKBR3 and MDAMB231 cancer cell lines, were enhanced by complexation. The anti-proliferative effects on the human lung cancer cell line were accompanied by cellular ROS generation and an increase in cytoplasmcondensation. The breast cancer cell lines did not produce caspase3/7 activation, mitochondrial potential reduction and ROS generation. Therefore, a non-apoptotic form of cell death in a caspaseand oxidative stress-independent manner has been proposed. The protein binding ability has been monitoredby the quenching of tryptophan emission in the presence of the compounds using bovine serum albumin (BSA) as a model protein. Both compounds could be distributed and transported in vivo and the complex displayed stronger binding affinity and higher contributions to the hydrogen bond and van der Waals forces.