CEQUINOR   05415
CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
artículos
Título:
Vanadium and cancer treatment: Antitumoral mechanisms of three oxidovanadium(IV) complexes on a human osteosarcoma cell line
Autor/es:
LEÓN I.; BUTENKO N; DI VIRGILIO A.L.; MUGLIA C.I.; BARAN, E. J.; CAVACO I; ETCHEVERRY, S.
Revista:
JOURNAL OF INORGANIC BIOCHEMISTRY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 134 p. 106 - 117
ISSN:
0162-0134
Resumen:
We report herein the antitumor actions of three oxidovanadium(IV)
complexes on MG-63 human osteosarcoma cell line. The three complexes:
VO(oda), VO(oda)bipy and VO(oda)phen (oda=oxodiacetate), caused a
concentration dependent inhibition of cell viability. The
antiproliferative action of VO(oda)phen could be observed in the whole
range of concentrations (at 2.5μM), while VO(oda)bipy and VO(oda)
showed a decrease of cell viability only at higher concentrations (at
50 and 75μM, respectively) (p<0.01). Moreover, VO(oda)phen caused a
decrease of lysosomal and mitochondrial activities at 2.5μM, while
VO(oda) and VO(oda)bipy affected neutral red uptake and mitochondrial
metabolism at 50μM (p<0.01). On the other hand, no DNA damage
studied by the Comet assay could be observed in MG-63 cells treated
with VO(oda) at 2.5-10μM. Nevertheless, VO(oda)phen and VO(oda)bipy
induced DNA damage at 2.5 and 10μM, respectively (p<0.01). The
generation of reactive oxygen species increased at 10μM of VO(oda)phen
and only at 100μM of VO(oda) and VO(oda)bipy (p<0.01). Besides,
VO(oda)phen and VO(oda)bipy triggered apoptosis as determined by
externalization of the phosphatidylserine. The determination of DNA
cleavage by agarose gel electrophoresis showed that the ability of
VO(oda)(bipy) is similar to that of VO(oda), while VO(oda)(phen) showed
the highest nuclease activity in this series. Overall, our results
showed a good relationship between the bioactivity of the complexes and
their structures since VO(oda)phen presented the most potent antitumor
action in human osteosarcoma cells followed by VO(oda)bipy and then by
VO(oda) according to the number of intercalating heterocyclic moieties.