CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Unidad Ejecutora - UE
Copper(II) complexes with cyanoguanidine and o-phenanthroline: Theoretical studies, in vitro antimicrobial activity and alkaline phosphatase inhibitory effect.
JUAN J. MARTÍNEZ MEDINA; LIBERTAD L. LÓPEZ TÉVEZ; NORA B. OKULIK; MARÍA S. ISLAS; EVELINA G. FERRER; PATRICIA A.M. WILLIAMS
JOURNAL OF MOLECULAR STRUCTURE
ELSEVIER SCIENCE BV
Lugar: Amsterdam; Año: 2014 vol. 1058 p. 298 - 298
Calculations based on density functional methods are carried out for two Cu(II) complexes with cyanoguanidine (cnge) and o-phenanthroline (o-phen): [Cu(o-phen)2(cnge)](NO3)2_2H2O (1) and [Cu(o-phen)(cnge)(H2O)(NO3)2] (2). The calculated geometrical parameters are in agreement with the experimental values. The results of Atoms in Molecules (AIM) topological analysis of the electron density indicate that the Cu?N(phen) bonds in complex (1) have lower electron density, suggesting that those bonds are stronger in complex (2). Moreover, the ionic character of the Cu?N bond in the complex (1) is slightly stronger than that in the complex (2) and this situation would explain the fact that only complex (2) was stable in water solution. For this reason, the antimicrobial and enzymatic assays were performed using complex (2). It is well known that the increased use of antibiotics has resulted in the development of resistant bacterial and fungal strains. In this context, the study of novel antimicrobial agents has an enormous importance and metal complexes represent an interesting alternative for the treatment of infectious diseases. The aim of this work is to prove the modification of some biological properties like antimicrobial activity or alkaline phosphatase inhibitory activity upon copper complexation. The antimicrobial profile of the metal, the ligands and complex (2) was studied against several bacterial and fungal strains by different microbiological methods. The values of MIC indicate that the complexation increases the antibacterial activity against Enterococcus faecalis, but decreases this activity against Pseudomonas aeruginosa. The complex (2) exhibited longer PAEs/PAFEs than copper and o-phen against Escherichia coli and all fungal strains, and longer PAEs/PAFEs than some antibiotics or antifungal agents against E. coli, Staphylococcus aureus and Candida albicans. Complexation also improves the alkaline phosphatase inhibitory effect of copper and cnge. Therefore, the interaction of copper(II) with N-containing ligands may provide a promising strategy for the development of novel drugs with enhanced antimicrobial activity or alkaline phosphatase inhibitory activity.