IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A rol for arachidonic acid derived endocannabinoids in Caenorhadbitis elegans dauer diapause regulation
Autor/es:
PREZ, G.M.; PENKOV, S.; KURZCHALIA, T.; PORTA, E.O.J.; LABADIE, G.R.; GALLES, C.; BOLAND, S.; DE MENDOZA, D.
Lugar:
Montevideo
Reunión:
Congreso; "Expanding Caenorhabditis elegans research: first Latin American worm meeting"; 2017
Institución organizadora:
Institut Pasteur
Resumen:
Polyunsaturated fatty acid (PUFAs) are important membrane components and precursor of different endocannabinoid, among other metabolites. To investigate the role that endocannabinoid (eCB) molecules derived from arachidonic acid such 2-arachidonoylglycerol (2-AG) play in C. elegans dauer diapause, we constructed a daf-7:fat-3 double mutant, which is deficient in the production of 2-AG, and we found that this strain has percentage of dauer formation than daf-7. Moreover, this strain can also overcome dauer arrest by 2-AG supplementation. We also show, using the HPLC-MS/MS techniques, that this eCB is indeed synthesized by wild type and daf-7 mutant that were grown in a depleted cholesterol media. These results indicate that 2-AG represents a signal that coordinates the status of long-chain fatty acid synthesis with metabolic changes, probably through the Cholesterol metabolism that ultimately determine the decision between diapause and reproduction in C. elegans.