Characterization of a new Baeyer-Villiger monooxygenase

ROMINA D. CECCOLI; DARIO BIANCHI; DANIELA V. RIAL

Viena

Congreso; 12th Biotrans; 2015

Universisdad Tecnológicade Viena

The oxidation of ketones mediated by Type I Baeyer-Villiger monooxygenases (BVMOs) is a key reaction for obtaining lactones or esters as versatile intermediates towards the synthesis of biologically active compounds and natural products. These enzymes depend on FAD and require NADPH as electron donor to catalyze the monooxygenation of the carbonyl substrate in the presence of molecular oxygen. Due to their strict dependence on NADPH for activity, biotransformations in whole-cell systems represent a valuable experimental strategy for synthetic applications.Aiming at expanding the biocatalytic tools for practical and green oxidation of ketones, we cloned and characterized a Type I BVMO detected in the proteome of Leptospira biflexa. The phylogenetic analysis based on known Type I BVMOs indicates that our new BVMO would belong to a clade that remains unexplored for biocatalysis. In addition, we predicted its probable three-dimensional structure by homology modeling and examined its substrate profile by biotransformations in recombinant whole-cells expressing this BVMO.