ANALYSIS OF DIFFERENT METHODOLOGIES FOR THE ENCAPSULATION OF SUPEROXIDE DISMUTASE INTO CHITOSAN MICROPARTICLES.

SALOMON C.; CAVIGLIA M.; CORTEZ N.; GOOYCOLEA F. ; RIVERA G.; HOFFMANN S

Conferencia; ICCC / EUCHIS 2015-12th International Conference of the European Chitin Society; 2015

The aim of this work was to analyze different techniques to encapsulate superoxide dismustase (SOD) into chitosan (CH) microparticles. SOD-CH microparticles were prepared through the ionotropic gelation process. Different solutions of CH, (85.5% DD; MW 239.2 KD) (1, 2 or 3% w/v) were dissolved in acetic acid solution to reach a final solution of pH 4, pH 5, or pH 6. A solution of sodium sulphate (5 ml, 20% w/v) was prepared. In the experiment A, SOD was added to CH solution. Then, to the SOD-CH solution, sodium sulphate solution was added dropwise through a no. 4 syringe needle under magnetic stirring at room temperature for 15 min, which led to the formation of microparticles. In the experiment B, SOD was added to the sodium sulphate solution. Then, the CH solution was added to the SOD-sodium sulphate solution, under the same conditions than experiment A. In addition, other modifications on the addition of the polymer solution to the sulphate solution were carried out. SOD activity was measured in acrylamide gel incubated with NBT, Riboflavin and EDTA. In addition, SOD activity was determined based on the inhibition of NBT reduction using the system xanthine/xanthine oxidase. The characterization of the SOD microparticles was done by SEM. The encapsulation efficacy was between 45 and 85% depending the chosen methodology. The yield was around 80-85% in all cases. It was found that the release of SOD from CH microparticles was directly related with the CH concentration and also with the pH of the solution.