IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IN VITRO CHARACTERIZATION OF SUPEROXIDE DISMUTASE (SOD)-LOADED MICROPARTICLES ON FIBROBLASTS
Autor/es:
CLAUDIO SALOMON; RAMANZIN FABRICIO; MAGDALENA LASTRA; NESTOR CORTEZ; F. FONTANA; G. SANDRI; CARLA CARAMELLA
Reunión:
Congreso; III Reunión Internacional de Ciencias Farmacéuticas RICIFA 2014; 2014
Resumen:
Superoxide dismutase is an enzyme counteracting Oxygen Reactive Species (ROS) in cells, thus preventing damage to tissues. Topical delivery of SOD to treat skin and mucosal deseases/injuries could benefit from the embedding of SOD into polymeric microparticles with the aim of improving stability and controlling drug release. The aim of this work was the evaluation of biocompatibility and antioxidant activity of Manganese-SOD chitosan-based microparticles onto a fibroblast cell line. Two protocols were developed to formulate SOD into non-crosslinked (sample 1) and crosslinked chitosan microparticles (sample 2). In the case of sample 1, SOD was dispersed into an acidic chitosan solution and the mixture was spray dried under specific conditions. Sample 2 was prepared by adding the SOD into a sodium sulphate solution. Then, this mixture was poured into the acidic chitosan solution while stirring. The microspheres were separated by centrifugation. The morphology and surface of the microparticles was study by SEM and the yield and encapsulation efficacy was also evaluated. SOD-microparticles were tested onto a bNHDF cell line and cell viability was assessed by MTT test after 24 h of incubation. A preventive oxidative test was then conducted on sample 1 onto bNHDF fibroblast to evaluate the protective effect (microparticles left in contact for 24h before insult) against the oxidative damages produced by hydrogen peroxide (incubation with 2 mM H2O2 for 24 h) chosen as model oxidant substance. The cytotoxicity results clearly show that SOD microparticles have the same viability as the complete medium, although dependent on the concentration used, and no significant differences are shown between the two different samples. The antioxidant activity test performed on sample 1, and evaluated at different dilutions (1:5; 1:10; 1:20; 1:40) in 10% FBS DMEM (standard medium), demonstrated that the 1/40 dilution was able to produce a significant antioxidant activity against H2O2.