New second-order luminescent signals for analyte quantitation in the presence of interferents: time-decay sensitized lanthanide excitation and time-evolving chemiluminescence

ALEJANDRO OLIVIERI, VALERIA LOZANO, GABRIELA IBAÑEZ Y ARSENIO MUÑOZ DE LA PEÑA

Praga, República Checa.

Congreso; XIV International Symposium on Luminescenc Spectrometry; 2010

ISLS

The determination of the antibiotic ciprofloxacin in urine and serum samples has been carried out in the presence of uncalibrated interferences, by processing new second-order signals, achieving the so-called second-order advantage. These are, respectively, lanthanide sensitized excitation-time decay matrices and the time evolution of chemiluminescence emission. Due to significant interactions between analyte and biological background, quantitation was performed in the standard addition mode, using a new processing method involving subtraction of the unknown data matrix from the standard addition matrices. In both cases, complete spectral overlapping between interferents and calibrated components occurs in one of the data dimensions, and thus only multivariate curve resolution-alternating least squares (MCR-ALS) and parallel factor analysis adapted for linear dependency (PARALIND) could be successfully applied to the studied data. The results indicate good analytical performance towards the analyte, with root mean square errors of ca. 10 and 0.4 mg/L in urine and serum respectively.