INTERACTION OF THE COMPLEX [Ag(SULFADIMETOXINATE)] WITH BOVINE SERUM ALBUMIN (BSA)

MONTI, L.; HURE, E.; MOSCONI, N.; PONTORIERO, A.; NERLI, V.; PICÓ, G.; ATRIA, ANA M.; CAMPAGNOLI, D.; RIZZOTTO, M.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2010 vol. 34 p. 78 - 78

0327-9545

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} a:link, span.MsoHyperlink {color:blue; text-decoration:underline; text-underline:single;} a:visited, span.MsoHyperlinkFollowed {color:purple; text-decoration:underline; text-underline:single;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Interaction of the complex [Ag(sulfadimetoxinate)] with bovine serum albumin (BSA) 1Monti, Laura; 1Hure, E.; 1Mosconi, N.; 1Pontoriero, A.; 2Nerli, B.; 2Picó, G.; 3Atria, Ana M.; 4Campagnoli, D.; 1Rizzotto, M.* Áreas 1Inorgánica y 2Fisicoquímica, FCByF, UNR, Rosario, Argentina; 3Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile; 4Facultad de Ingeniería Química, UNL, Santa Fe, Argentina.  E-mail: rizzotto@iquir-conicet.gov.ar     Binding of drugs to plasma proteins is one of the factors that affect their availability in the human body. Formation of complexes between sulfa drugs and metallic ions is an extended field of research because it improves the action of the sulfa drug. In the present work we studied the interaction of BSA with the complex Ag-sulfadimetoxine (Ag-SDM). When aqueous solutions of silver nitrate and sodium sulfadimetoxine (NaSDM) were mix a white solid was obtained, which was separated and washed, in different experiences, with water or water and methanol. In both cases the compound was solved in DMSO and later purified through crystallization in a methanol camera. Elemental analysis (N, C, H, S and Ag) let us suggest that all the solids were the same compound, with the following formula: [Ag(C12H13N4O4S]. In order to study the interaction of Ag-SDM with BSA, aqueous solutions of BSA with and without ligand (Ag-SDM and initial drugs) were analyzed by UV-Vis spectroscopy. An increment in absorbance and shifts to shorter wavelengths were found with NaSDM and AgSDM. It was found a saturation effect with NaSDM and a possible cooperative interaction with AgSDM. In conclusion, the formation of the complex AgSDM could be produce changes in the mechanism of binding of NaSDM to BSA