Why lamivudine assembles into double-stranded helices in crystals: salt heterosynthon versus basepairing homosynthon

MEDANHA VALDO, A. K.; SAROTTI, A.M.; CHAPELETTI DA SILVA, C.; RIBEIRO, L.; DO NASCIMENTO NETO, J. A.; MARTINS, F. T.*

CRYSTENGCOMM

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2018 vol. 20 p. 3049 - 3057

1466-8033

Here we were interested in obtaining a better understanding of the competition between the salt hetero synthon and the base-pairing homosynthon formed by the anti-HIV drug lamivudine in the presence of strong acids. Even though the preparation of the multicomponent crystal forms of this drug with weak ormoderate acids is well investigated, the crystallization of lamivudine with strong acids is still little explored. Besides filling this crystallization screening gap, the driving forces of the so-called lamivudine duplexescould be drawn from the complete structural landscape and the theoretical thermodynamic parameters of the salt heterosynthon, calculated at the M06-2X/6-31+G** level of theory. Five new crystal structures of lamivudine were obtained, wherein two of them were assembled as base-paired helically-stacked strandsin the presence of trifluoroacetic and trichloroacetic acids (lamivudine duplexes V and VI, respectively). Besides, three salts were prepared by crystallization of lamivudine with sulfuric and perchloric acids. Finally,the theoretical approach showed that there is no energy trend regarding the formation of lamivudine duplexes with aliphatic organic acids or lamivudine salts with aromatic acids, which is usually observed in practice.