Enantiomeric analysis of overlapped chromatographic profiles with presence of interferences. Application to the enantiomeric composition determination of ibuprofen in a pharmaceutical formulation containing homatropine

ARANCIBIA, J.; CASTELLS, C. B.; ARANCIBIA, J.; CASTELLS, C. B.; OSORIO GRISALES, J.; CASTELLS, C. B.; OSORIO GRISALES, J.; CASTELLS, C. B.; J. M. PADRÓ; OLIVIERI, A.; J. M. PADRÓ; OLIVIERI, A.

JOURNAL OF CHROMATOGRAPHY - A

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 1467 p. 255 - 260

0021-9673

Ibuprofen is widely used as a non-steroidal anti-flammatory drug. Wedemonstrated that the combination of chemometric methods withchromatographic-spectroscopy data can be a powerful analyticalstrategy in the analysis of enantiomers when complete separation isnot possible or requires very long analysis times. In the presentwork, the determination of ibuprofen enantiomers in pharmaceuticalformulations containing homatropine as interference by chiralHPLC-DAD detection and in combination with partial least squarealgorithms is proposed. The methodhas been applied to samples containing enantiomeric ratios from 95:5to 99.5:0.5 and coelution of interferents. The results were validatedusing univariate calibration and without homatropine. Relative errorof the method was less to 4.0%, for both enantiomers. Limits ofdetection and quantification for (S)-(+)-ibuprofenwere 4.96×10-10and 1.50×10-9mol, respectively (when theinjection volume was 10 µL).Finally, the chemometricmethod was applied to the determination of enantiomeric purity ofcommercial pharmaceuticals. The ultimate goal of this research wasthe development of rapid, reliable, and robust methods for assessingenantiomeric purity by conventional diode array detector assisted bychemometric tools.