IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
artículos
Título:
A Multivariate Approach for the Simultaneous Determination of Losartan Potassium and Hydrochlorothiazide in a Combined Pharmaceutical Tablet Formulation
Autor/es:
RUBÉN M. MAGGIO; PATRICIA M. CASTELLANO; TEODORO S. KAUFMAN
Revista:
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Editorial:
Springer
Referencias:
Año: 2008 vol. 391 p. 2949 - 2955
ISSN:
1618-2642
Resumen:
A convenient new method for the simultaneous determination of losartan potassium and hydrochlorothiazide, with minimum sample pre-treatment, is described. The procedure, based on the multivariate analysis of spectral data in the 220 - 274 nm region by the partial least squares algorithm, is linear in the concentration range 1.06 - 5.70 mg L-1 for hydrochlorothiazide and 4.0 - 22.2 mg L-1 for losartan. It is simple, rapid and robust, allowing accurate and precise results, with drug recovery rates of 99.3 and 100.4 % and relative standard deviations of 1.7 and 1.0 %, for hydrochlorothiazide and losartan, respectively. The method was applied to the simultaneous determination of both analytes in tablets, providing good results, which were in statistical agreement with those provided by independent HPLC analyses of the samples. The method has also been successfully applied for the construction of drug dissolution profiles of a commercial pharmaceutical preparation containing both analytes.