Chemopreventive action of ascorbic acid on the mutagenicity of sulfathiazole-nitrite reaction mixtures in acidic medium: preliminary studies

PONTORIERO, A.; TROSSERO, C.; GORR, C.; HURE, E.; MONTI, L.; MOSCONI, N.; CAFFARENA, G.; RIZZOTTO, M.

BIOCELL

Año: 2008 vol. 32 p. 18 - 18

0327-9545

  <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES-AR;} a:link, span.MsoHyperlink {color:blue; text-decoration:underline; text-underline:single;} a:visited, span.MsoHyperlinkFollowed {color:purple; text-decoration:underline; text-underline:single;} @page Section1 {size:595.3pt 841.9pt; margin:5.0cm 183.9pt 359.4pt 6.0cm; mso-header-margin:35.45pt; mso-footer-margin:35.45pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> CHEMOPREVENTIVE ACTION OF ASCORBIC ACID ON THE MUTAGENICITY OF SULFATHIAZOLE-NITRITE REACTION MIXTURES IN ACIDIC MEDIUM: PRELIMINARY STUDIES 1Pontoriero, Ana; 1Trossero, Claudia; 1Gorr, Carolina; 1Hure, Estela; 1Monti, Laura; 1Mosconi, Natalia; 2Caffarena, Graciela; 1Rizzotto, Marcela* 1Área Inorgánica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Suipacha 531; 2Cátedra de Microbiología, Facultad de Ciencias Médicas, Av. Francia y Santa Fe. UNR, 2000 Rosario  E-mail: mrizzott@fbioyf.unr.edu.ar  Sulfonamides -or their derivatives- are widely used by their properties (antibacterial, antifungal, antidiabetic, herbicides, analytical reagents, etc.). One risk of sulfas is the possible formation of mutagenic products by reaction with nitrite in stomach. Ascorbic acid (Asc.) can react with nitrite, diminishing or removing the nitrosation risk. According to the Ames test a compound would be a mutagen if the reversion coefficient, R.C. (R.C.: revertant number per tested plate/revertant number per control plate) is ≥ 2. Previously we proved mutagenic action of reaction mixtures of sodium sulfathiazole and NaNO2 in acidic medium with the S. typhimurium TA98 strain. In the present work it was proved the chemopreventive action of one dose of Asc. on the mutagenicity of such reaction mixture. Different doses of the reaction mixture were assayed in the presence of a constant dose of Asc. with the TA98 strain. In all cases the R.C. was 1 ± 0.20. Using the following equation: % inhibition of mutagenicity = (1 - R.C. of the reaction mixture+Asc./ R.C. of the reaction mixture without Asc.).100, it was obtained a 66% of inhibition which, as a preliminary level, suggests a strong inhibitory effect.