Discriminant analysis of Annona muricata and Rollinia mucosa extracts by multivariate curve resolution and partial least-squares regression of liquid chromatography-diode array data

AFONSO, S.; PISANO, P. L.; SILVA F. B.; SCAMINIO, I. S.; OLIVIERI, A. C.

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY

SOC BRASILEIRA QUIMICA

Lugar: San Pablo; Año: 2015 vol. 26 p. 2241 - 2248

0103-5053

Species of the Annonaceae family are used in traditional medicine, and several antitumoracetogenins have been isolated from the leaves of Annona muricata and Rollinia mucosa.Leaves samples (self-shaded and sun-exposed) of these species were collected during winter and summer seasons. Extraction media were prepared from mixtures of five solvents according to a simplex centroid design. Plant extracts were analysed by injection into a high-performance liquid chromatography with diode array detection (HPLC-DAD) and evaluated after applying by multivariate curve resolution-alternating least-squares (MCR-ALS) and discriminant-unfolded partial least-squares (D-UPLS). The objective was to check whether the chemometric resolution of chromatographic profiles could help in the discrimination of samples according to taxonomic classification, sun exposition and/or harvest season. The results indicate that extraction media containing ethanol as main solvent achieved discrimination of self-shaded versus sun-exposed plants and harvest season of Annona muricata. Also, D-UPLS analysis allowed taxonomic discriminationbetween Annona muricata and Rollinia mucosa extract samples.