IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Loading of Cells with Fluorescent Probe to Study Intracellular Acid-base Homeostasis in Lactic Acid Bacteria
Autor/es:
MORTERA, P.; ZULJAN, F.; MAGNI, CH.; ALARCON, S.
Revista:
Bio-protocol
Editorial:
Stanford University
Referencias:
Año: 2015 vol. 2
ISSN:
2331-8325
Resumen:
Here we describe a protocol which we have used to study the homeostasis intracellular in vivo in Lactic Acid Bacteria (LAB) using a fluorescent probe. The use of these probes for determiningchanges in the pH cytoplasm with high sensitivity, temporal resolution and ease of use to assess the rate of change of cell pH in responseto a stimulus from kinetic measurements on short time scales (Breeuwer et al., 1996; Molenaar et al., 1991). This protocol has been designed to measure the in-vivo intracellular pH using the pH-sensitive fluorescent probe 2´,7´-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) in LAB, Enterococcus faecalis (E. faecalis), Lactococcus lactis (L. lactis) and Lactobacillus casei (L. casei).