IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Determination of pesticides and metabolites in wine samples by HPLC-DAD and second-order calibration methods
Autor/es:
ALEJANDRO CESAR OLIVIERI; HÉCTOR GOICOECHEA,; J. POPPI, RONEI; B. BRAGA, JEZ W.; G. BOTTOLI, CARLA B.
Revista:
JOURNAL OF CHROMATOGRAPHY - A
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2007 vol. 1148 p. 200 - 210
ISSN:
0021-9673
Resumen:
The
second-order calibration models PARAFAC and the recently introduced BLLS were
applied to several chromatographic-diode array detection systems, where overlap
of interferences with the compounds of interest was observed, making the
determination and resolution of the analytes possible. In this work, the
simultaneous determination of five pesticides and two metabolites in wine
samples by HPLC-DAD was performed, using the so-called second-order advantage.
The results of two chromatographic methods were compared, involving either
isocratic or gradient elution. An appropriate preprocessing method was
necessary to correct the effects of time shifts, baseline variations and
background. BLLS presented results that were of the same quality as PARAFAC in
five cases, but in two other situations only PARAFAC enabled analyte
quantitation. Relative errors of prediction lower than 10 % for all compounds
were obtained, indicating that the methodology employing HLPC-DAD and
second-order calibration can handle complex analytical systems.