IQUIR   05412
INSTITUTO DE QUIMICA ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Determination of pesticides and metabolites in wine samples by HPLC-DAD and second-order calibration methods
Autor/es:
ALEJANDRO CESAR OLIVIERI; HÉCTOR GOICOECHEA,; J. POPPI, RONEI; B. BRAGA, JEZ W.; G. BOTTOLI, CARLA B.
Revista:
JOURNAL OF CHROMATOGRAPHY - A
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2007 vol. 1148 p. 200 - 210
ISSN:
0021-9673
Resumen:
The second-order calibration models PARAFAC and the recently introduced BLLS were applied to several chromatographic-diode array detection systems, where overlap of interferences with the compounds of interest was observed, making the determination and resolution of the analytes possible. In this work, the simultaneous determination of five pesticides and two metabolites in wine samples by HPLC-DAD was performed, using the so-called second-order advantage. The results of two chromatographic methods were compared, involving either isocratic or gradient elution. An appropriate preprocessing method was necessary to correct the effects of time shifts, baseline variations and background. BLLS presented results that were of the same quality as PARAFAC in five cases, but in two other situations only PARAFAC enabled analyte quantitation. Relative errors of prediction lower than 10 % for all compounds were obtained, indicating that the methodology employing HLPC-DAD and second-order calibration can handle complex analytical systems.