Partition in aqueous two-phase system: Its application in downstream

LUIS RODRÍGUEZ DURÁN; DARÍO SPELZINI; VALERIA BOERIS; CRISTÓBAL AGUILAR; GUILLERMO PICÓ

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 101 p. 392 - 397

0927-7765

Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by  polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found  to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition  suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom   phase due to the high molar mass of the enzyme. The process was significantly influenced by the   top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems,   a good performance was found, since the enzyme recovery in the bottom phase of the system composed  by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.Aspergillus niger was partitioned in aqueous two-phase systems composed by  polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found  to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition  suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom   phase due to the high molar mass of the enzyme. The process was significantly influenced by the   top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems,   a good performance was found, since the enzyme recovery in the bottom phase of the system composed  by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.