In vivo evaluation of albendazole microspheres for the treatment of Toxocara canis larva migrans

BARRERA, M. G.; LEONARDI, D.; BOLMARO, R. E.; ECHENIQUE, C. G.; OLIVIERI, A. C.; SALOMÓN, C. J.; LAMAS, M. C.

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Elsevier

Lugar: Amsterdan; Año: 2010 vol. 75 p. 451 - 454

0939-6411

Albendazole is a benzimidazole derivative with proven efficacy against many parasites such as intestinal helminths. Toxocariasis is one of the important parasitic diseases in humans and animals caused by Toxocara canis. It is well known that Toxocara canis larvae migrate in paratenic hosts, including humans where it may cause visceral larva migrans. Thus, the present research was carried out using in vivo experiments with the aim of finding if novel albendazole microparticles would be active against migrating larvae of the parasite. Albendazole-chitosan microparticles were prepared by ionotropic gelation with sodium lauryl sulphate or by a liquid-liquid phase separation with sodium hydroxide. Mice were infected with T. canis and then treated with both albendazole chitosan microparticles. After treatment (28 days post-infection), it was examined the anthelmintic effect in mice after oral administration of microparticulate preparations. The number of larvae recovered from mice treated with albendazole formulations were compared with placebo. The results showed that albendazole microparticles were easily prepared in high yield using both aqueous solutions of sodium lauryl sulphate or sodium hydroxide. In vivo evaluation of larva migration showed that albendazole microparticles exhibited a greater anthelmintic effect in the brain (0 larva/mouse). In addition it was also found that liver and lung showed a significant decrease in the number of larvae. Therefore, these data suggest that albendazole-chitosan microparticles are effective formulations for the treatment of toxocariasis infection by reducing the number of larvae in liver and lung. Particularly, these polymeric preparations were able to totally prevent migration of larvae to the mice brain exhibited a greater anthelmintic effect in the brain (0 larva/mouse). In addition it was also found that liver and lung showed a significant decrease in the number of larvae. . It is well known that Toxocara canis larvae migrate in paratenic hosts, including humans where it may cause visceral larva migrans. Thus, the present research was carried out using in vivo experiments with the aim of finding if novel albendazole microparticles would be active against migrating larvae of the parasite. Albendazole-chitosan microparticles were prepared by ionotropic gelation with sodium lauryl sulphate or by a liquid-liquid phase separation with sodium hydroxide. Mice were infected with T. canis and then treated with both albendazole chitosan microparticles. After treatment (28 days post-infection), it was examined the anthelmintic effect in mice after oral administration of microparticulate preparations. The number of larvae recovered from mice treated with albendazole formulations were compared with placebo. The results showed that albendazole microparticles were easily prepared in high yield using both aqueous solutions of sodium lauryl sulphate or sodium hydroxide. In vivo evaluation of larva migration showed that albendazole microparticles exhibited a greater anthelmintic effect in the brain (0 larva/mouse). In addition it was also found that liver and lung showed a significant decrease in the number of larvae. Therefore, these data suggest that albendazole-chitosan microparticles are effective formulations for the treatment of toxocariasis infection by reducing the number of larvae in liver and lung. Particularly, these polymeric preparations were able to totally prevent migration of larvae to the mice brain exhibited a greater anthelmintic effect in the brain (0 larva/mouse). In addition it was also found that liver and lung showed a significant decrease in the number of larvae.