CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression profile analysis of malic enzyme transcripts during different stress condition in maize
Autor/es:
ALVAREZ, CLARISA; SAIGO, MARIANA; ANDREO, CARLOS; DRINCOVICH, MARÍA FABIANA
Lugar:
Puerto Madryn
Reunión:
Congreso; SAIB. XLVI Reunión Anual; 2010
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Resumen:
Malic enzymes (ME): two plastidic NADP-MEs (ZmME1 andZmME2); three cytosolic NADP-MEs (ZmME3, ZmME4 andZmME7); and two mitochondrial NAD-MEs (ZmME5 andZmME6). To elucidate the role of each ME, maize plants wereexposed to different stress conditions, like UV light; rootincubation with Na CO pH 8 or 11; or cellulose. After the 2 3treatments, the expression level of each ME transcript, along withME activity, were analyzed. After UV treatment, a five-timeincreased expression of ZmME1 was found. Meanwhile, arepression of two-times was observed for ZmME3. ZmME2increased three-times in leaves by incubation with Na CO pH 8, 2 3while it was repressed two-times in roots. At pH 11, this gene wasinduced 4-times in roots but repressed in the same level in stems.The gene for ZmME7 was repressed five-times at pH 8. Rootincubation with cellulose, increased 4-times ZmME4 and ZmME7.Finally, the transcripts encoding NAD-ME (ZmME5 and ZmME6)did not significantly changed in any of the treatments evaluated,suggesting that mitochondrial NAD-ME might be involved inmetabolic pathways not related with these type of stress. Overall,the results obtained indicate that each ME fulfils particular roles invivo, probably related to the specific kinetic properties andmetabolic regulation of each isoform.