CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of frataxin homologs in plants
Autor/es:
TUROWSKI, V.; CARRILLO, M; LEADEN, L; BUSI, M. V.; DIEGO FABIAN GOMEZ CASATI
Lugar:
Puerto Madryn
Reunión:
Congreso; XLVI Reunion SAIB; 2010
Institución organizadora:
SAIB
Resumen:
Frataxin is a highly conserved protein from
bacteria to mammals that has been proposed to participate in iron-sulfur
cluster assembly, mitochondrial iron homeostasis and defense against oxidative
stress. In eukaryotes, frataxin is encoded in the nucleus but has mitochondrial
localization. Recently, we reported the presence of a single gene that codify this
protein in Arabidopsis thaliana (AtFH); while three different genes has
been predicted to codify frataxin isoforms in maize (ZmFH EU968030, BT040175 y BT055005).
We cloned and characterized two of these isoforms (EU968030 and BT055005). Our
results indicate that these isoforms are functional and suggest that both have
a similar role in maize. We also found that frataxin homologs interact with the
mitochondrial cysteine desulfurase AtNfs1 using a pull-down assay. In addition,
frataxin increases the catalytic efficiency of AtNfs1, suggesting a direct role
of frataxin in the modulation of sulfide transfer into the scaffold proteins
Isu and HscB.