Comparative proteomics of Prunus persica leaves exhibiting leaf peach curl disease

VELENTINI, H; NOVELLO, A; LARA, MARÍA V.; DRINCOVICH, M. F.

Congreso; LIV Reunión SAIB; 2018

Prunuspersica (L.) Batsch is a species of great economic importance. Several conditions affects its production; among them, the biotrophicfungus Taphrina deformans (Berk.) Tul. causes the leaf peach curl disease. Symptoms include chlorosis, pink pigmentation and leaf curling. Inorder to study the effect of the infection in leaves proteome, symptomatic leaves exhibiting the disease (50% of area thickening and curving)were collected from naturally infected trees. The leaves were processed by separating the symptomatic area (C) and the asymptomatic (N) region.Asymptomatic healthy leaves (G0) were also collected. Quantitative proteomics were performed using total extracted proteins and liquidchromatography/mass spectrometry. Differential proteins were identified using the Perseus software platform. A total of 1725 proteins wereidentified in leaves. Comparison of N with G0 identified only ninety proteins, while C against G0 or N, rendered 664 and 232 proteins,respectively. Data obtained revealed a repression of the protein synthesis machinery, a repression of the primary metabolism and photosynthesis,plus a turnover and reorganization of the cell wall, alterations in the secondary metabolism and an increase in the content of pathogen responseproteins in G7C in comparison with G0. Complementary biochemical analysis including quantification of total protein content, Western blot ofRubisco and proteins involved in primary metabolism, and chlorophyll, anthocyanins and flavonoids quantitation, were conducted in order tovalidate the results obtained