CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CHARACTERIZATION OF A FEMALE GAMETOPHYTIC MUTANT IN Arabidopsis thaliana
Autor/es:
IGLESIAS MJ; TOSSI V; GERGOFF G; FANELLO D; LARRAMBURU E; GONZALEZ ME; LOBATO C; TUROWSKI V; VILLARREAL V; GÓMEZ-LOBATO ME; MITTON F; MANSILLA Y; REUTEMANN A; GONORAZKY G; PAGNUSSAT G; ZABALETA E
Lugar:
San Miguel de Tucumán, Tucumán. República Argentina
Reunión:
Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)
Resumen:
CHARACTERIZATION OF A FEMALE GAMETOPHYTIC MUTANT IN Arabidopsis thaliana
These authors contributed equally to the present work.
Development and function of gametophytes are crucial for plant
reproduction. However, relatively little is known about the
molecular basis of embryo sac development. We characterized a
Ds insertion line (MEE 42) with defects in female transmission
efficiency. The phenotype of MEE 42 was studied by DIC
microscopy of whole-mount preparations of ovules. Mutant ovules
were found to be affected during post-pollination processes.
Embryo development was arrested at one-cell zygotic stage.
Endosperm development was also arrested. Some ovules appeared
unfertilized, suggesting that the mutation is also affecting pollen
tube (PT) guidance or fertilization. To further study the nature of
the fertilization failure, we analyzed PT growth patterns by aniline
blue staining. Although pollen tubes were found at the micropyle of
both WT and mutant ovules, some mutant ovules showed abnormal
PT growth patterns. To characterize the identity of the synergide
cells -responsible for PT attraction- we tested the expression of a
synergide marker. Similar GUS staining patterns were observed in
WT and MEE42 mutant embryo sacs. The flanking sequence of the
Ds element was confirmed for the 5 end of the DS element by
using Ds and specific primers to be in At3g63080, encoding a
glutathione peroxidase.