OPTIMIZED TECHNIQUE APPLIED TO THE DETERMINATION OF AMINO ACID CONTENT IN FOOD AND BIOLOGICAL HUMAN FLUIDS

MULLER G.L.; ANDREO C. S.; WIGGENHAUSER J.; DRINCOVICH M. F.; ALVAREZ C.E.; LARA M.V.

Rosario

Congreso; Second american metabolic profiling symposium; 2016

Recent studies have indicated that amino acid analysis is a fundamental analytical technique, which can be used for the determination of amino acid composition in the content of proteins, peptides and other pharmaceutical or biological sample fractions. Specifically, since proteins and various amino acids are needed in the human diet to promote protein synthesis and amino acid circulation, amino acid analysis can be used to monitor or detect the metabolic states by analyzing the content of free amino acids in biological fluids such as urine, blood or plasma. Also, the determination of amino acid content is an essential information provided in the label of food products. In this research we optimized the use of a reverse Phase C18 HPLC of derivatized amino acids which provides highly reproducible quantitative analysis of samples for the estimation of amino acid composition in specific samples such as human blood or tomato samples. Such technique uses internal standards of known amount to gain reliable quantitative results, enabling the accurate quantification of each amino acid. Most importantly, this technique is dedicated to drive the variety of amino acid analysis methods with more effective applications. As an integral part of analytical biochemistry, amino acid analysis is continuously showing its functions on scientific studies.