Lipid profiling of peach leaves from genotypes with contrasting susceptibility to Taphrina deformans

GOLDY; CAMILA; FERNIE, ALISDAIR R; BROTMAN, YARIF; VALENTINI, GABRIEL; LARA, MARÍA VALERIA; BUSTAMANTE, CLAUDIA; SVETAZ, LAURA; DRINCOVICH, MARÍA FABIANA

Córdoba

Congreso; LII Reunión Anual de SAIB; 2016

Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

Peach Leaf Curl disease, caused by T. deformans, affects yield and plant longevity. Only a few cultivars are considered tolerant. We conducted a lipidomic analysis of healthy leaves from a resistant (DR) and a susceptible (DS) genotype, and of asymptomatic DS leaves in which the pathogen was detected (DS+). By using ultra-performance liquid chromatography coupled to Fourier-transform mass spectrometry, we detected 132 lipids. Principal component analysis revealed the lipidome of DR clearly differs from that of DS, and that of DS significant changes upon T. deformans infection. DR shows higher levels of MGDG 34:5 (37%), MGDG 34:6 (33%), TAG 60:3 (16%), PI, PC PE, PG and SQDG and lower levels of DAG 36:3 (8%), DAG 36:6 (7%) and MGDG 36:2 (8%) than in DS. Comparing infected and healthy leaves of DS, while the behavior is variable within each member of the different groups of lipids (TAGs, DAGs, SQDGs and phospholipids), the levels of MGDG is not affected and those of DGDG decrease in infected leaves. Galactolipids are important constituents of chloroplastic membranes and have also associated with the medicinal and nutritional properties of vegetable plants. Here, results obtained suggest that galactosyldiacylglycerol lipids (MGDG and DGDG) may have a critical role in the response of Prunus persica against T. deformans.