Analysis of metabolic integrators from Nannochloropsis gaditana

BARCHIESI, J.; HEDIN, N.; PEREYRA, D; BUSI, M.V.

Cordoba

Congreso; LII Congreso Anual de la Sociedad Argentina de Investigación Bioquímica; 2016

Keeping up with demand for food, fuels and biological products of interest require an urgent effort on increasing crop yields in a sustainable way. Photosynthetic microorganisms suchas eukaryotic microalgae possess several advantages over higher plants, higher growth rate, high photosynthetic conversion efficiencies, increased lipid and carbohydrateaccumulation, and ease of manipulation are just a few. Nannochloropsis gaditana is a promising production microalga whose published genome consists of 30 chromosomes and isunder 30Mb in total. It has high nutritional value with elevated protein and polyunsaturated fatty acid (PUFA) content. Lipid accumulation accounts for more than half of its biomass. Onthe other hand, transcript profiling of carbon regulated-genes from Arabidopsis thaliana identified a myo-inositol-1-phosphate synthase gene, whose expression correlates withincreased biomass. In this work we assayed heterogeneous growth conditions of Nannochloropsis gaditana and analyzed the differential yields on protein content, hydro-carbons andlipids amounts as well as photosynthetic pigments in comparison to the expression of the algal myo-inositol-1-phosphate synthase gene.