Metabolomic response of soybean leaves to Fusarium tucumaniae

ROSATI, R.; SCANDIANI, M.M.; CERVIGNI, G.; LUQUE, A.; SPAMPINATO, C.

Córdoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica (SAIB); 2016

SAIB

Sudden-death syndrome(SDS) of soybean can be caused at least by 4 different Fusarium species: F.brasiliense, F. crassistipitatum, F. tucumaniae and F. virguliforme, with F.tucumaniae being the dominant specie in Argentina. Infection and diseasedevelopment are highly dependent on environmental conditions. Argentineansoybean germplasm show various levels of resistance to F. tucumaniae. Here, two soybean cultivars, one susceptible (S) andone partially resistant (R) to SDS were inoculated (I) with an isolate of F. tucumaniae grown in sorghum grain,using the layer method. Uninoculated controls (C) were included for both genotypes.Plants were grown for 7 days. To detect early metabolic markers that couldpotentially discriminate between S and R genotypes, leaf metabolite profilingby gas chromatography mass spectrometry was performed. Compounds wereputatively identified by comparison of their retention index and mass spectrumwith those present in the commercial mass spectra library NIST. These analysesidentified amino acids, organic acids, soluble sugars, alcohols, fatty acidsand miscellaneous compounds. Metabolite levels were normalized to the ribitolinternal standard and compared between samples. Levels of two soluble sugars(psicose and rhamnose), glycerol and isothiocyanate were increased afterinfection and this increment was dependent on the genotype.