CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
UV-B radiation inhibits cell proliferation in maize leaves through transcriptional changes of cell cycle and developmental genes
Autor/es:
GERRIT BEEMSTER; FINA, JULIETA;; PAULA CASATI; CASADEVALL, ROMINA;
Reunión:
Congreso; XXXI Reunión Argentina Fisiología Vegetal; 2016
Resumen:
Previously, we showed that UV-B radiation inhibits A. thaliana (a dicot plant) growth by inhibiting cell division in proliferating leaves. UV-B also affects maize (Zea mays, a monocot) growth. To analyze this phenotype, we exposed maize seedlings to UV-B from germination until the completion of leaf 4 expansion. Maize plants grown in the presence of UV-B showed a 15% reduction in final leaf size. Leaf 4 elongation rate was 15% lower in leaves exposed to UV-B. A kinematic analysis of leaf growth rates in control and UV-B-treated leaves was also done for a detailed spatial and temporal characterization. Epidermal cells expanded to the same mature cell length but cell production decreased by 36% in UV-B-treated leaves; while average cell cycle duration was not affected. These results suggest that overall postmeristematic cell expansion is unaffected by UV-B and that reduced cell production is responsible for the observed growth reduction. RNA-seq was done to characterize the transcriptome of the growing zone of control and UV-B exposed plants to shed light on the molecular pathways involved in UV-B inhibition of leaf growth. Our results show a link between the observed leaf growth inhibition and the expression of specific cell cycle and developmental genes.