CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of the mechanism of stone hardening during the development of peach fruit
Autor/es:
RODRIGUEZ, CARLOS; BUSTAMANTE C.; BUDDE, C; LARA, MARÍA VALERIA; MARIA FABIANA DRINCOVICH
Reunión:
Congreso; LI Reunión de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015
Resumen:
Lignification of peach (Prunus persica) fruit endocarp involves a strictly coordinated process, which consists of lignin biosynthesis, deposition in secondary cell wall and programmed cell death. Understanding the mechanisms and regulations involved in lignin synthesis and the formation of stone in the development of endocarp in peach fruit, is relevant not only scientifically but also economically by the benefits that could come off in terms of fruit and biofuel production. In the present work, proteomic studies were performed in order to identify differential protein expression during early stages of peach fruit development. By using two-dimensional differential gel electrophoresis, 30 differentially expressed proteins among the E1 (10 DAB), S1 (30 DAB) and S2 (50 DAB) proteome samples were detected. From them, 14 proteins increased along the development, while 16 proteins decreased from E1 to S2 stages. Detected proteins include polyphenoloxidases and ABA/WDS induced proteins, among others. Furthermore, the level of expression of some enzymes involved in the phenylpropanoid pathway, which results in anthocyanin synthesis and precursors of lignin, was analyzed by qRT-PCR in endocarp, mesocarp and exocarp tissues obtained from the three stages. Overall, the present study may contribute to understand the molecular basis for stone formation during early peach fruit development.