CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of Arabidopsis SINA-L7, a potential mediator of a RRM and its interaction with GAPC.
Autor/es:
PERALTA, DA; PEROTTI V; BUSI, M. V.; DIEGO FABIAN GOMEZ CASATI
Lugar:
Rosario
Reunión:
Congreso; . L Reunión de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2014
Institución organizadora:
SAIB
Resumen:
Arabidopsis Seven in absentia like 7 (SINA-L7), belonging to the seven
in absentia family is a putative Zinc finger (SIAH-like) nuclear-cytosolic
protein that is expressed in various growth stages and almost all plant tissues.
Its homolog was first identified in Drosophila
associated with the development of photoreceptors, cell cycle arrest and
protein polyubiquitination. We found SINA-L7
expression altered in different plant lines with mitochondrial dysfunction as
for example in gapc-1 KO plants. The glycolytic enzyme GAPC was also found in
the nucleus and associated to the mitochondrial external membrane in plants, thus,
it may have other important roles. However, little is known about these
possible secondary functions in higher plants. In this context, we face the
characterization of SINA-L7 and study its interaction with GAPC in vitro. Results show that SINA-L7 is highly expressed in tissues with
active cell division and it is induced also after UV exposure. Furthermore, using
an ubiquitination assay we found that SINA-L7 has E3-ubiquitin ligase activity and that the K124 residue is
involved in such process. In addition, SINA-L7 ubiquitinates and physically
interacts with GAPC by pull down analysis and residue K231 in GAPC is responsible
of its interaction. Furthermore, residue K76 in GAPC is ubiquitinated by
SINA-L7, and this modification regulates GAPC activity.