Glycine rich RNA binding proteins in tomato fruits: a novel positive regulation of expression

MÜLLER, GABRIELA LETICIA; TRIASSI, AGUSTINA; LARA, MARÍA VALERIA; DRINCOVICH, MARÍA FABIANA; ANDREO, CARLOS SANTIAGO

Mendoza

Congreso; XLVIII Reunión Anual de SAIB; 2012

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

In this study, the analysis of GRP1 gene family was undertaken inMicro-Tom tomato fruit. Three genes ( ) wereidentified which are highly homologous to each other and to GRPsof related species. Three transcriptional products for each genewere identified, the un-spliced pre-RNA, the maturemRNAand thealternative spliced mRNA ( , and). LeGRPs show the classical N-terminal end RNArecognition motif and the C-terminal end glycine-rich region,suggesting conservation of functional properties. The levels of alltranscriptional forms of and the total immunoreactiveLeGRP1 decrease during tomato fruit development from immaturegreen to red ripe. Particular circadian profiles of expression weredetected for each transcriptional form. During ripeningoff the vine after mature green, the levels of all transcriptionalforms increased at 20ºC but not at 4ºC. The correlationanalysis of the expression of all transcriptional formssuggests a novel model of positive regulation of expression intomato fruit, in which each immature form ofpositively correlates with each other and the formcorrelates depending on the stage. The results obtained reveal acomplex pattern of expression of GRP in tomato fruits suggestingthese proteins as key regulators of important processes in fruits.