CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
capítulos de libros
Título:
Malate and fumarate emerge as key players in primary metabolism:Arabidopsis thaliana overexpressing C4-NADP-ME offer a way tomanipulate the levels of malate and to analyse the physiological consequences
Autor/es:
FAHNENSTICH, H.; SAIGO, M; NIESSEN, M.; ANDREO, C.S.; DRINCOVICH, M.F.; FLUGGE, U.; MAURINO , VG
Libro:
Photosynthesis. Energy from the Sun
Editorial:
Allen, Grantt and Osmond, eds. Springer
Referencias:
Año: 2008; p. 845 - 849
Resumen:
Maize C4-NADP-malic enzyme was expressed under the control of the CaMV 35S
promoter in Arabidopsis thaliana. An increase in the plastidic NADP-ME activity
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
promoter in Arabidopsis thaliana. An increase in the plastidic NADP-ME activity
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
4-NADP-malic enzyme was expressed under the control of the CaMV 35S
promoter in Arabidopsis thaliana. An increase in the plastidic NADP-ME activity
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
Arabidopsis thaliana. An increase in the plastidic NADP-ME activity
induced no phenotypic differences in long-day growth conditions. Analysis of metabolite
levels revealed a disturbed metabolic profile. Dark-induced senescence progressed more
rapidly in MEm plants compared to the wild-type. A retardation of senescence in the
transgenic lines was gained by exogenous supply of glucose, sucrose and malate,
suggesting that the lack of a rapid energy source is likely to be the initial factor leading to
the induction of senescence in these plants. A fairly complete picture of primary
metabolism assessed by GC-MS and the in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
in vitro metabolic complementation assays allow
us to conclude that MEm transgenic plants entered dark induced senescence more rapidly
due to an accelerated starvation. Comparison of the data obtained indicated that
extremely low levels of malate and fumarate are responsible for the accelerated darkinduced
senescence encountered in the MEm plants. Reinforcing previous results, our
data indicate that malate and fumarate are key players in the primary metabolism of
Arabidopsis thaliana..