CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
Identification and characterization of a novel Kazal-type serine proteinase of Arabidopsis thaliana.
Autor/es:
ROSSI, F.; SIMON, F.; DIEGO FABIAN GOMEZ CASATI; CLEMENTE, M.; PARIANI, S.; ACOSTA, D.M.; CORIGLIANO, M.G.; PIECKENSTAIN, F.; CONTRERAS, M.; SANDER, V.; BUSI, M. V.; DUSCHAK, V.
Revista:
BIOCHIMIE
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Año: 2016 vol. 123 p. 85 - 94
ISSN:
0300-9084
Resumen:
Many different types of serine proteinase inhibitors have been involved in several kinds of plant physiologicalprocesses, including defense mechanisms against phytopathogens. Kazal-type serine proteinaseinhibitors, which are included in the serine proteinase inhibitor family, are present in several organisms.These proteins play a regulatory role in processes that involve serine proteinases like trypsin, chymotrypsin,thrombin, elastase and/or subtilisin. In the present work, we characterized two putative Kazaltypeserine proteinase inhibitors from Arabidopsis thaliana, which have a single putative Kazal-typedomain. The expression of these inhibitors is transiently induced in response to leaf infection byBotrytis cinerea, suggesting that they play some role in defense against pathogens. We also evaluated theinhibitory specificity of one of the Kazal-type serine proteinase inhibitors, which resulted to be inducedduring the local response to B. cinerea infection. The recombinant Kazal-type serine proteinase inhibitordisplayed high specificity for elastase and subtilisin, but low specificity for trypsin, suggesting differencesin its selectivity. In addition, this inhibitor exhibited a strong antifungal activity inhibiting the germinationrate of B. cinerea conidia in vitro. Due to the important role of proteinase inhibitors in plantprotection against pathogens and pests, the information about Kazal-type proteinase inhibitorsdescribed in the present work could contribute to improving current methods for plant protectionagainst pathogens.