CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
A simple method for the addition of rotenone in Arabidopsis thaliana leaves.
Autor/es:
MALIANDI, M.V.; RIUS, S.P.; BUSI, M.V.; GOMEZ-CASATI, D.F
Revista:
PLANT SIGNALING & BEHAVIOR
Editorial:
TAYLOR & FRANCIS
Referencias:
Lugar: Filadelfia; Año: 2015
ISSN:
1559-2324
Resumen:
A simple and reproducible method for the treatment of Arabidopsis thaliana leaves with rotenone is presented. Rosette leaves were incubated with rotenone and Triton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, 2 genes known to be induced in Arabidopsis cell cultures after rotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented an inhibition of oxygen uptake. The simplicity of the procedure shows this methodology is useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.