CEFOBI 05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
A simply method for the addition of rotenone in Arabidopsis thaliana leaves
Autor/es:
MALIANDI, M.V.; RIUS, S.P. (CO-PRIMER AUTOR); BUSI, M.V.; GÓMEZ-CASATI, D.F.
Revista:
Plant Signaling and Behavior
Editorial:
Taylor & Francis
Referencias:
Lugar: Philadelphia, PA; Año: 2015
ISSN:
1559-2316
Resumen:
A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
